Course unit code:
N418L10_4B
Course unit title:
Laboratory of Instrumental Methods in Analysis
Mode of delivery, planned learning activities and teaching methods:
laboratory practice – 3 hours weekly (on-site method)
Credits allocated:
3
Recommended semester:
Biotechnológia – bachelor (full-time, attendance method), 5. semester
Chemical Engineering – bachelor (full-time, attendance method), 5. semester
Potraviny, výživa, kozmetika – bachelor (full-time, attendance method), 5. semester
Automation, Information Engineering and Management in Chemistry and Food Industry – bachelor (full-time, attendance method), 5. semester
Level of study:
1.
Prerequisites for registration:
none
Assesment methods:
Practical execution of all laboratory exercises and test.
Learning outcomes of the course unit:
Provide advanced work using laboratory analytical techniques, basics of proper implementation of the analytical operations, good laboratory practice. Know the principles of creation of analytic signal measuring instruments and principles of optimization of various modes of measurement. Control instrumental analytical procedures for obtaining information and procedures evaluation of measurement results.
Course contents:
1.Basic principles work in the analytical laboratory . Working with measures of weight and
volume. Preparation of solutions. Titration technique.
2.Optical methods - the absorption methods of determination by the addition of standard and
nephelometric / turbidimetric methods of determination by the calibration line, the emission
spectrum methods. Identification of organic substances on the basis of a comprehensive
interpretation of spectra NMR, MS,IR.
3.Electroanalytical methods - voltammetry as analytical technique, study electrochemical
processes and potentiometric titration of acid mixtures. Derivative and Granov transform of
the titration curves. Polarography and biampermetrická titration, precise preparation of
the buffer solutions and pH measurement.
4.Chromatography techniques - liquid chromatography and gas chromatography in
quantitative analysis, the internal standard method and preconcentrate treatment techniques
of the samples, SPE. Liquid and gas chromatography as an identification method.
5.Test.
Recommended or required reading:
Basic:
  • PURDEŠOVÁ, A. – TARAPČÍK, P. – SÁDECKÁ, J. – HROBOŇOVÁ, K. – ŠPÁNIK, I. – RIEVAJ, M. – ŠVORC, Ľ. – MANOVÁ, A. – ČACHO, F. Praktikum z analytickej chémie. Bratislava : Nakladateľstvo STU, 2011. 242 p. ISBN 978-80-227-3555-1.
  • BUSTIN, D. – HLADKY, Z. – GARAJ, J. Analytická chémia. Alfa, Bratislava: SNTL, Praha, 1987. 744 p.
Language of instruction:
Slovak, English
Assessed students in total:
209

A 25.8 %

B 34 %

C 30.6 %

D 8.6 %

E 1 %

FX 0 %

Course supervisor:
Ing. Alena Manová, PhD.
Last modification:
16. 1. 2018

Department:
Department of Analytical Chemistry

AIS: 2018/2019   2017/2018  

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