ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Kinetic-spectrophotometric approach to the modified Berthelot procedure for serum urea determination

Ivana D. Rašić Mišić, Snežana S. Mitić, Danijela A. Kostić, Snežana B. Tošić, Emilija T. Pecev-Marinković, and Ana S. Miletić

Department of Chemistry, Faculty of Sciences and Mathematics, University of Niš, Niš, Serbia

E-mail: ivana.rasic@pmf.edu.rs

Received: 18 January 2020  Accepted: 11 August 2020

Abstract:

The results of developed kinetic-spectrophotometric method for serum urea determination by modified colorimetric Berthelot procedure were presented in this paper. To obtain high sensitivity, all reaction rates were monitored spectrophotometrically by measuring the change of absorbance with time at 700 nm. A differential variant of the tangent method was used for the processing of the kinetic data based on linear dependence of absorbance on time. The method is valid over the 0.25–2.50 µg cm⁻³ urea concentration interval with RSD range 8.33–2.02%. The limit of detection was calculated as 0.09 µg cm⁻³. The interference effects of some metal ions, anions, antibiotics, and other molecules were investigated to access the method selectivity. The method was successfully applied for the determination of urea in human quality control blood serum.

Keywords: Kinetics; Urea; Modified Berthelot procedure; UV/Vis spectrophotometry; Human blood serum

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-020-01315-x

Chemical Papers 75 (2) 565–574 (2021)