ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

A red-emitting fluorescent probe for visualizing mitochondrial microviscosity by cell imaging

Yeming Zhang, Bingsong Xu, Hao Chen, Bin Fang, Hui Wang, and Lei Hu

College of Pharmacy, Wannan Medical College, Wuhu, China

E-mail: wanghias@126.com

Received: 1 September 2020  Accepted: 7 January 2021

Abstract:

The visual detection of mitochondrial viscosity is of considerable significance in that it is associated with various cellular functions. Herein, a novel red-emitting viscosity probe YD-Mito constructed by linking phenylamine derivatives and benzo[e]indolium through a C–C double bond has been designed and synthesized. It was found that YD-Mito exhibited a special response to viscosity accompanying with a sharp fluorescence enhancement (20-fold) at 620 nm. Importantly, YD-Mito can precisely localized in mitochondria independent from membrane potential and visualized the mitochondrial viscosity. Therefore, probe YD-Mito could provide a powerful tool for the determination of mitochondrial viscosity.

Keywords: Viscosity; Mitochondrial; Cell imaging; TICT

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-021-01504-2

Chemical Papers 75 (6) 2517–2523 (2021)