ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Implementation of mature tea leaves extract in bioinspired synthesis of iron oxide nanoparticles: preparation, process optimization, characterization, and assessment of therapeutic potential

Md Salman Haydar, Dipayan Das, Suravi Ghosh, and Palash Mandal

Nanobiology and Phytotherapy Laboratory, Department of Botany, University of North Bengal, Siliguri, India

E-mail: pmandalbotppprl@nbu.ac.in

Received: 12 June 2021  Accepted: 9 September 2021

Abstract:

In recent years, the investigation of quick, efficient, and green method of metal nanoparticles synthesis has gained considerable importance in various dimensions of nanotechnology. But there are certain limitations to this emerging interest assize, morphology, and bioactivity of nanoparticles produced through green synthesis often varies greatly corresponding to the specific condition of metallic precursor and reducing agent. Current study intends to explore optimum condition like concentration of metallic precursor, plant extract (PLX), their volumetric ratio during biogenic synthesis of iron oxide nanoparticles (FeNPs) using aqueous extracts of mature tea leaves which is basically a waste product with no commercial importance and generally discarded after pruning of young leaves and buds. The study also deals with the characterization of nanoparticles synthesized at optimized condition, investigation of antimicrobial and antioxidant propensity of the same. The optimal reactant concentration for biosynthesis of FeNPs was claimed to be10 mM FeCl_3, 100 mg/mL plant extract and volumetric ratio of FeCl₃:PLX = 10:1. The FeNPs obtained through this route had a spherical to irregular morphology with crystalline nature, average TEM and hydrodynamic size of 13.09 and 75.25 nm, respectively, having a zeta potential value of + 46.2 mV indicating strong stability. Synthesized FeNPs was found to be effective against wide range of soil microbes with highest activity against gram-negative bacteria (Escherichia coli) than gram-positive bacteria. Biosynthesized nanoparticles showed dose dependent antioxidant activity against all the tested parameters with highest against DPPH and least active against nitric oxide.

Keywords: Iron oxide nanoparticles; Green synthesis; Process optimization; Antimicrobial activity; Antioxidant potentiality; Therapeutic potentiality

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-021-01872-9

Chemical Papers 76 (1) 491–514 (2022)