ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

AuNPs-/c-MWCNT-/GQDs-modified electrode-based genosensor for early diagnosis of rheumatic heart disease

Ankur Kaushal, Ashok Kumar, Deepak Kala, Shagun Gupta, and Dinesh Kumar

Shoolini University of Biotechnology and Management Science, Solan, India

E-mail: chatantadk@yahoo.com

Received: 18 January 2022  Accepted: 3 March 2022

Abstract:

In the present work, a specific DNA sensor has been fabricated by immobilizing 5’-NH₂-labeled single-stranded DNA probe (ssG-DNA) specific to S. pyogenes on AuNPs-/c-MWCNT-/GQDs-based screen-printed electrode. Further, hybridization studies were performed using different concentrations of DNA isolated from bacteria and swab samples from susceptible patients. Electrode surface modifications were characterized using scanning electron microscopy (SEM). Electrochemical response in terms of cyclic voltammetry (CV) and differential pulse voltammetry (DPV) was recorded and analyzed to validate DNA hybridization. The limit of detection (LOD) and the sensitivity of the developed sensor were found to be 0.2 pg/µL and 1095.2 µAcm^− 2 ng⁻¹, respectively. The sensor was found stable for 6 months with 9–10% loss in initial DPV current at 4 °C. The sensor is highly specific, simple, and sensitive with patient swab samples and takes only 20 min for detection of S. pyogenes infection.

Keywords: S. pyogenes; AuNPs; MWCNT; DNA sensor; Rheumatic heart disease

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02163-7

Chemical Papers 76 (7) 4621–4626 (2022)