ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Microbial remediation of ibuprofen contaminated water using novel isolate Microbacterium paraoxydans

Sumona Show, Priyanka Sarkar, Sanghamitra Barman, and Gopinath Halder

Chemical Engineering Department, National Institute of Technology Durgapur, Durgapur, India

E-mail: gopinath.halder@che.nitdgp.ac.in

Received: 22 April 2022  Accepted: 15 September 2022

Abstract:

Ibuprofen (IBP), a commonly recommended nonsteroidal, antipyretic, analgesic and anti-inflammatory drug, is a widespread ecological contaminant with harmful consequences. This issue has necessitated the development of a comprehensive scientific strategy for the removal of drugs as well as their by-products from the environment. Biodegradation is a method for breaking down complex chemicals into simpler forms that is both eco-friendly and effective. The current research studies the biodegradation of IBP by isolating a promising bacterial strain isolated from a pharmaceutical industrial wastewater. Microbacterium paraoxydans (Genbank accession numbers: Forward OL614700 and Reverse OL614701) was identified as the bacterium strain by 16S rRNA sequencing. Enhancement of IBP biodegradation efficiency was attained by optimization of parameters using central composite design (CCD). Due to their critical impact on IBP degradation, five parameters were selected for optimization: pH, yeast extract, temperature, inoculum dose, incubation time, and agitation speed. The other two parameters, concentration and time, were kept unchanged. At ideal conditions of pH 7, inoculum dose OD₆₀₀, agitation speed of 150 rpm, 30 °C temperature, and 0.3% yeast extract, the target drug concentration was reduced by 92.01%. The results suggest that Microbacterium paraoxydans could be an efficient microbe for degrading pharmaceutical wastewater and reducing harmful environmental effects.

Keywords: Ibuprofen; Biodegradation; Optimization; Pharmaceutical wastewater; Growth kinetics

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02499-0

Chemical Papers 77 (1) 517–531 (2023)