ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

β-galactosidase as an industrial enzyme: production and potential

Rahul Vikram Singh and Krishika Sambyal

Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India

E-mail: rahul.negi121@gmail.com

Received: 28 May 2022  Accepted: 20 September 2022

Abstract:

β-galactosidase is the principal enzyme for dairy and pharmaceutical industries owing to its multiple applications. Different sources of β-galactosidase such as plants, microbes, mammals have been explored by far. Due to its high industrial demands, recombinant strains have also been developed and well-characterized for industrial applications apart from the already existing natural sources. Immobilization strategies such as entrapment, cross-linking, covalent binding and physical absorption are also performed to increase the stability and durability, therefore reducing the process cost. This review provides systematic information about the recent advancements in the production of β-galactosidase and its industrial applications. Recombinant strains are observed to be dominant in the industry along with immobilization strategies and used predominantly for prebiotic galactooligosaccharides production. These developments on novel β-galactosidases derived from microbial sources or by engineering means together have converted the enzyme into a relevant synthetic tool with the use of efficient recombinant systems.

Keywords: β-galactosidase; Lactose intolerance; Recombination; Immobilization; Transglycosylation

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02507-3

Chemical Papers 77 (1) 11–31 (2023)