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Features of synthesis of sodium and carbonate containing biphasic calcium phosphates and their cytotoxicity

N. Strutynska, M. Slobodyanik, T. Tykhonenko, Y. Titov, and N. Stus

Chemistry Department, Taras Shevchenka National University of Kyiv, Kiev, Ukraine

 

E-mail: Strutynska21.05@gmail.com

Received: 27 June 2022  Accepted: 26 September 2022

Abstract:

Biphasic calcium phosphates (BCP) (hydroxyapatite/β-tricalcium phosphate = HAP/β-TCP) were synthesized from aqueous solutions in different weight ratios of components containing 0.5–2 wt % of Na+ and up to 3 wt % of CO32−. According to powder XRD data, the increasing of Na+ amount in the initial solution (molar ratio Na+/Ca2+  = 0.02–0.1) led to growing of the content of phase based on β-Ca3(PO4)2 (TCP—up to 90wt%) while the addition of carbonate-anions (CO32−/PO43− = 0.1) caused to increase of apatite-type phase content (up to 90wt%) in the obtained compositions. Cytotoxicity of synthesized biphasic calcium phosphates was determined by MTT test. Cells were incubated with different amount (0.05–20 mg/ml) of composites for 24 h. It was found that addition of 20 mg/ml of biphasic calcium phosphate with ratio HAP/β-TCP = 90/10 wt% decreased cell viability of 3T3 cells (the embryonal mouse fibroblast cell line) by only 20% while presence of sample with ratio HAP/β-TCP = 10/90wt% led to an enhancement in optical density which could be a result of an increase in the number of cells or in cell density. The found conditions for preparation of biphasic calcium phosphates in different weight ratios (HAP/β-TCP) containing different amounts of sodium cations (0.5–2%wt) and up to 3%wt of carbonate can be further used in the development of materials with the required resorption rate for orthopedics.

Keywords: Biphasic calcium phosphates; Whitlockite; Hydroxyapatite; FTIR spectroscopy; Cytotoxicity

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02521-5

 

Chemical Papers 77 (2) 805–812 (2023)

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