ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Au, Ag nanoparticles-doped MIL-53(Fe) in rapid and selective detection of hydrogen peroxide in milk samples

Prince George and Pradip Chowdhury

Department of Chemical Engineering, National Institute of Technology Rourkela, Rourkela, India

E-mail: chowdhuryp@nitrkl.ac.in

Received: 28 June 2022  Accepted: 19 October 2022

Abstract:

This work reports the successful incorporation of Au and Ag nanoparticles within MIL-53(Fe) matrix which showed excellent morphological stability and dispersive capability as confirmed via various techniques. Au@MIL-53(Fe), Ag@MIL-53(Fe) were employed as a nanozyme (specifically mimicking peroxidase-like activity) for colorimetric detection of H₂O₂. The reaction mechanism of peroxidase-like activity was traced via radical studies, and hypothetical reaction routes were formulated for a better understanding of TMB (colorless) oxidation to oxo-TMB (blue). The study showed marked improvement in H₂O₂ detection with LOD of 0.0623 µM within a linear range (1–10 µM) (S/N = 3). The presence of H₂O₂ in spiked milk samples was analyzed and modeled through linear regression, and the resulting model was validated with an accuracy of 0.465–0.45% with RSD% lying within 1.93–3.81% for two different models falling within linear ranges (1–10 µM) and (10–100 µM), respectively. The value of activation energy for Au@MIL-53(Fe) was ca. 41.94 kJ.mol⁻¹ which was found to be lower than pristine MIL-53(Fe) [ca. 49.36 kJ.mol⁻¹]. Overall, Au@MIL-53(Fe) demonstrated an excellent colorimetric sensor for the detection of H₂O₂ both in model solution and in processed milk and could trace possible adulteration of milk. The material holds promise in the fabrication of various diagnostic prototypes for specific applications in food processing, and medical and environmental remediation.

Keywords: Metal nanoparticles; MOFs; Peroxidase-like activity; H2O2 sensor; Microwave synthesis

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02558-6

Chemical Papers 77 (3) 1361–1375 (2023)