ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Preparation of N, S-doped blue emission carbon dots for dual-mode glucose detection with live cell applications

Marimuthu Amarnath and Chinnasamy Balalakshmi

Department of Nanoscience and Technology, Alagappa University, Karaikudi, India

E-mail: samyasribala@gmail.com

Received: 31 October 2022  Accepted: 4 March 2023

Abstract:

In this present work, we have developed a dual-mode colorimetric and photoluminescent sensor based on nitrogen and sulphur-doped carbon dots (NSCDs) by cost-effective and simple hydrothermal method. Citric acid and glutathione were taken as the initial precursors. These NSCDs have shown excellent physiochemical and optical properties with high quantum yield. Here, it is more attractive one that when the prepared NSCDs were binded with glucose, the colour of the solution changes from colourless to blue under direct sunlight without the aid of an external ultraviolet lamp. Blue emission behaviour of these NSCDs was utilized for the selective binding confirmation and quantification of glucose. For selectivity, test various biomolecules have been analysed and the results showed high selectivity towards glucose molecules. 10 µM Glc showed emission of bright blue fluorescence with limit of detection at 0.26 μM concentration, and it was given by the photoluminescence behaviour based on 3σ/s criteria. This strategy has been applied for the visual detection of glucose in HepG2 cell which offers innovative perception into the biomedical applications of NSCDs to develop a way to sense glucose in clinical analysis.

Keywords: NSCDs; Colorimetric; Photoluminescent; Glucose; HepG2 cells

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-02769-5

Chemical Papers 77 (8) 4193–4199 (2023)