ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Rapid and sensitive colorimetric detection of ascorbic acid using carboxymethyl cellulose functionalized Co₃O₄ nanoparticles as peroxidase mimic

Shehala, Puneet Tiwari, Tulika Malviya, and Vandana Singh

Department of Chemistry, University of Allahabad, Prayagraj, India

E-mail: singhvandanasingh@rediffmail.com

Received: 27 March 2023  Accepted: 17 May 2023

Abstract:

Herein we demonstrate the synthesis, characterization, and peroxidase-mimicking behavior of carboxymethyl cellulose-Co₃O₄ nanoparticles (C@NZ₃) toward rapid and sensitive colorimetric detection of ascorbic acid (AA). AA was detected by monitoring the quenching of color signal that was obtained from C@NZ₃ catalyzed H₂O₂ oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The excellent peroxidase-mimicking behavior of C@NZ₃ is evidenced by the low detection limit of AA (0.41 μM) and good linearity in the range of 0.5–30 μM. The method is cost-effective, highly selective, and requires only 3 min to complete. C@NZ₃ exhibited high shelf life and could be successfully used to detect AA in real samples of human serum.

Keywords: Carboxymethyl cellulose; Co3O4; Nanoparticles; Ascorbic acid; Sensing

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-02876-3

Chemical Papers 77 (9) 5441–5453 (2023)