ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Utility of eco-friendly microwave-assisted nitrogen-doped carbon dots as a luminescent nano-sensor for the ultra-sensitive analysis of tigecycline in dosage form and biological samples

Baher I. Salman

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt

E-mail: bahersalman@azhar.edu.eg

Received: 24 January 2023  Accepted: 4 June 2023

Abstract:

In the presented work, simple, green, sensitive, and selective nitrogen-doped carbon quantum dots (N-CQDs) were developed as nano-sensor for quantification of tigecycline (TIG) in different matrices. The proposed method is based on microwave synthesis of green nitrogen-doped carbon quantum dots with a high quantum yield (41.39%) and size diameter equal to 2.0 nm from the green juice of Eruca sativa leaves. The relative fluorescence intensity (RFI) of the green synthesized quantum dots (N-CQDs) was quenched at emission 512 nm (excitation 445 nm) after the addition of TIG drug. A good linear range between TIG concentration and quenched fluorescence intensity of N-CQDs in the range 20–300 ng mL⁻¹, with the lower limit of quantitation (LOQ) equal to 8.51 ng mL⁻¹. The proposed method was validated using the international conference of harmonization (ICH) recommendation and bio-analytical validation using U.S. food and drug administration (US-FDA) guidelines. The N-CQDs have been fully characterized using a transmission electron microscope (TEM), dynamic light scattering (DLS), X-Ray photoelectron spectroscopy (XPS), and Fourier-transform infrared spectrometer (FTIR). The suggested technique is a straightforward analytical procedure that can be used in clinical laboratories. Under the optimum condition, TIG was estimated in human plasma with a high percentage of recovery ranging from 96.95 to 98.54%. In addition, the proposed method was applied effectively in milk samples with percentage of recovery equal to 98.90 ± 1.55.

Keywords: Tigecycline; Plasma; Vial; N-CQDs; Fluorimetry; Milk

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-02914-0

Chemical Papers 77 (10) 5979–5988 (2023)