Magnetic solid phase extraction based on Fe3O4@SiO2@BZC as adsorbent for the extraction and pre-concentration of doxycycline hyclate from water and urine samples prior to UV–vis spectrophotometric analysisTabarak Mohammed and Hind Hadi Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq
E-mail: hind.h@sc.uobaghdad.edu.iq Received: 1 December 2023 Accepted: 6 April 2024 Abstract: This work describes a simple and straightforward magnetic solid phase extraction (MSPE) approach based on benzalkonium chloride-modified silica-coated magnetite nanoparticles (Fe3O4@SiO2@BZC). The developed extraction method was employed for extracting doxycycline hyclate (DOX) from water and urine samples after derivatization with procaine benzylpenicillin (PP) as a color reagent. An orange azo dye with a maximum adsorption wavelength (λmax) of 440 nm was produced by the azo-coupling of DOX with the color reagent in the alkaline medium. The analytical conditions were optimized carefully, including the reagent and surfactant volumes, the amount of sorbent, the extraction time, and the pH of the solution. The developed extraction approach achieved pre-concentration and enrichment factors of 47 and 25, respectively. UV–vis spectroscopy was utilized to determine the target analyte after the extraction step. A good linearity (R2 > 0.99) in the concentration range of 0.08–3.00 μg/mL was achieved. Detection and quantification limits of DOX were found to be 0.01 and 0.04 μg/mL, respectively. The suggested method was successfully applied to the extraction and determination of DOX spiked in urine and various water samples, with excellent recoveries of 97.00–98.30% and 98.00–102.00%, respectively. Keywords: Azo dye; BZC; Doxycycline hyclate; Extraction; Magnetic nanoparticles; Procaine penicillin Full paper is available at www.springerlink.com. DOI: 10.1007/s11696-024-03453-y
Chemical Papers 78 (8) 5071–5084 (2024) |
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