ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
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A new spectrophotometric method for the quantitative determination of Metopimazine based on the absorbance of its sulfoxide

Olena Mozgova, Mykola Blazheyevskiy, Liubomyr Kryskiw, Tetyana Kucher, Oksana Shliusar, and Valeriy Moroz

Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland

Received: 16 February 2024 Accepted: 6 June 2024

Abstract:

Metopimazine (MPZ) is a dopamine antagonist from the phenothiazine class of drugs. It has antiemetic properties and is used to treat nausea and vomiting, including those associated with cancer chemotherapy. We have proposed a new method for the quantitative determination of Metopimazine by spectrophotometry. The drug is determined using a difference spectrophotometric technique based on the absorbance of its previously prepared sulfoxide derivative (λ_max = 355 nm, ε = 4.5 × 10³ L/mol cm) relative to a solution of a non-derivatised drug. The sulfoxide derivative is formed quickly and quantitatively at room temperature by adding potassium caroate solution. The difference in the absorbance of solutions at 355 nm is proportional to the concentration of MPZ in the drug. The method is specific for an intact drug in the presence of oxidative and photochemical decomposition products and excipients. The linearity range is 0.9–30.0 μg/mL. Regression line equation: ΔA = 0.0061 × c (r = 0.999), where c is the MPZ concentration in μg/mL. The limit of quantitation, LOQ (10S), is 2.8 μg/mL. The possibility of quantitative determination of MPZ content in the bulk drug and tablets of Vogalen Lyok 7.5 mg with a relative standard deviation of 1.21% (δ = + 0.05%) and 1.38% (δ = – 0.67%), respectively, was shown. │δ│ < t_α (0.95, n = 7) × RSD/√n, where δ = (\(\overline{x}\)–μ) × 100%/μ (μ—quantification data, given in the Certificate of Quality).

Keywords: Metopimazine; Phenothiazine sulphoxide; Spectrophotometry; Pharmaceutical analysis; Quantitative determination; Oxone

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-024-03558-4

Chemical Papers 78 (11) 6585–6591 (2024)

Saturday, August 24, 2024