ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

In vivo biocompatibility of electrospun chitosan/PEO nanofiber as potential wound dressing material for diabetic burn wound injury of mice

Farhin Khan, Baishakhi Bairagi, Bidya Mondal, Dipankar Mandal, and Debjani Nath

Department of Zoology, University of Kalyani, Kalyani, Nadia, India

E-mail: nath_debjani@yahoo.co.in

Received: 27 February 2024  Accepted: 24 September 2024

Abstract:

Diabetes is a lifestyle havoc of the present epoch and patients suffer from all co-morbid situations. Diabetic burn wound patients are mostly affected due to the high rate of infection-alluring co-morbidity. Our study focused on the use of a unique combination of Chitosan/Polyethylene oxide (CS/PEO) woven into fibers by electrospinning to relieve these critical health conditions. The anti-inflammatory, antibacterial and tissue-repairing virtue of chitosan along with the broad-spectrum antibacterial effect of polyethylene oxide were highlighted for the treatment of ectopic infection and re-epithelialization of tissue. Single-dose streptozotocin-induced diabetic mice model was developed to study the applicability of CS/PEO nanofiber as dressing material on burn wounds. The average nanofiber diameter was 165  nm mimicking the extracellular matrix with a significant degree (125%) of swelling and thermal stability. Studies of optical density on the bacterial species identified from the wound suggested more than 50% antibacterial activity. Planimetric assessment, Haematoxylin & Eosin staining (HE) and Masson’s Trichrome (MT) staining suggested a remarkable 94.7% (p < 0.01) wound contraction with the increasing trend of re-epithelialization and  a reduced rate of inflammation in diabetic mice over 20 days. So, CS/PEO nanofiber can be used as an excellent alternative dressing material to heal diabetic burn wounds by limiting inflammatory infiltration and microvessels along with antibacterial efficacy.

Keywords: Diabetic; Chitosan; Polyethylene oxide; Burn wound; Healing; Nanofiber

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-024-03714-w

Chemical Papers 78 (16) 8833–8847 (2024)