ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

A sensitive guanine-based DNA-biosensor to monitor Cabozantinib anticancer in biological samples

Mohamed J. Saadh, Nadhir N. A. Jafar, Nizar Jawad, Farag M. A. Altalbawy, Ghassan Ali Dayeh, Mandeep Kaur, Ali G. Alkhathami, and Ali Altharawi

Faculty of Pharmacy, Middle East University, Amman, Jordan

E-mail: kaurmandeeep2000@gmail.com

Received: 11 February 2025  Accepted: 8 November 2025

Abstract:

The present article developed a highly sensitive DNA biosensor using modified screen-printed electrode (SPE) with polypyrrole (PP), praseodymium doped nanoneedle-assembled hierarchical NiO nanoflower (Pr³⁺/N-A H NiO NF) and ds-DNA to detect Cabozantinib as an anticancer drug. The ds-DNA/PP/Pr³⁺/N-A H NiO NF/SPE biosensor was created through a layer-by-layer fabrication process. Moreover, successful synthesis of Pr³⁺/N-A H NiO NF were verified by physicochemical characterization methods. The electrochemical impedance spectroscopy (EIS) revealed the ability of the modified SPE to reduce charge transfer resistance, resulting from the facilitated particle transfer and enhanced electrical conductivity. The biosensor accurately determined Cabozantinib within a range between 0.001 and 400.0 μM and LOD of 0.11 nM. It also effectively monitored Cabozantinib in real samples, with a recovery range between 98.0 and 100.8%.

Keywords: Cabozantinib; Anticancer; DNA-biosensor; Nickel oxide; Nanoflower

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-025-04513-7

Chemical Papers 80 (3) 2229–2243 (2026)