ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

GC-MS Determination of Fatty Acids in Arachidonic Acid High-Yield Strain Induced by Low-Energy Ion Implantation

X. Wang, J. Yao, and Z. Yu

Key Laboratory of Ion Beam Bioengineering of the Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, P. R. China

E-mail: xqwang@ipp.ac.cn

Received: 25 March 2004

Abstract: With a novel mutation technique, low-energy ion beam implantation, which was originated in our laboratory, an arachidonic acid high-yield strain ( Mortierella alpina ) was obtained. The mycelium of this strain was dried and treated with boron fluoride—methanol solution. The fatty acids were determined by gas chromatography—mass spectrometry. The result showed that there were saturated and unsaturated fatty acids in the dried mycelium. The relative content of arachidonic acid was 70.2 % among all fatty acids. In addition, there were linoleic acid (3.985 %) and γ -linolenic acid (1.576 %) in the dried mycelium.

Full paper in Portable Document Format: 594a240.pdf

Chemical Papers 59 (4) 240–243 (2005)