ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Physiological and Enzymatic Activities of Rhizopus oligosporus in Swing Solid-State Reactor

H. Miszkiewicz, A. Rozwandowicz, and S. Bielecki

Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, PL-90 924 Lodz

E-mail: hamisz@snack.p.lodz.pl

Received: 1 April 2004

Abstract: Hydrated, acidified, dehulled, and autoclaved Pisum sativum seeds were fermented with Rhizopus oligosporus NRRL 2710. Solid-state fermentations were carried out in a SWiNG-Solid-State-Reactor S-10-F and in Petri dishes. Effects of loading rates (23 %, 46 %, 58 %, and 70 %) and aeration intensity (5.0 dm³min⁻¹kg⁻¹, 7.5 dm³min⁻¹kg⁻¹, and 10.0 dm³min⁻¹kg⁻¹) on morphological and physiological characteristics of R. oligosporus NRRL 2710 and activity of its selected extracellular hydrolases were determined. Loading rate of 58 % and aeration of 10 dm³min⁻¹kg⁻¹ provided the highest physiological activity of the fungus and most intensive secretion of α-amylase, cellulase, lipase, and proteases.

Full paper in Portable Document Format: 586a424.pdf

Chemical Papers 58 (6) 424–428 (2004)