ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Isolation and Characterization of the Non-Starch Polysaccharides of Amaranth Seeds

A. Burisová, B. Tomášková, V. Sasinková, and A. Ebringerová

Department of Saccharides and Food Preservations, Faculty of Chemical Slovak University of Technology. SK-812 37 Bratislava Technology,

E-mail: burisova@chtf.stuba.sk

Abstract: The isolation of water-soluble polysaccharides (WSF) solubilized during separation of starch and protein from whole grain amaranth flour by wet sieving in a very mild alkaline medium as well as of polysaccharides (ASF), extracted from the insoluble cell wall material (ISCW-1) by hot dilute alkali, is described. The yields of the separated starch, protein, WSF, and ISCW-1, considered as the "fibre" component of amaranth, were 32.9, 29.3, 1.6, and 8.0 %, respectively, Sugar analysis of the isolated polysaccharide fractions in combination with FTIR spectroscopy indicates the presence of highly branched axabinoxylan and arabinogalactan, in addition to the prevailing nonseparated starch and protein. Glucuronoarabinoxylan was evidenced in ASF, but not in the WSF fractions.

Full paper in Portable Document Format: 554a254.pdf

Chemical Papers 55 (4) 254–260 (2001)