ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Synthesis of Ribonucleoside 5'-Triphosphates Derived from 2-Thiocytidine, 4-Thiouridine, and 5-Bromocytidine

B. Nawrot, P. Hoffmüller, and M. Sprinzl

Laboratorium für Biochemie, Universität Bayreuth, D-9544О Bayreuth, Germany

Abstract: The synthesis of 5'-triphosphates of 4-thiouridine, 2-thiocytidine, 5-bromocytidine, and [6-N-15]adenosine is described. All compounds are characterized by chromatographic and spectral data. Phosphorylation of corresponding nucleotides was performed using phosphoryl chloride and condensation with pyrophosphate. Intermediates were not isolated. The final products were purified by chromatography and characterized by ³¹P  NMR spectroscopy. The nucleoside triphosphates are substrates for RNA polymerase from bacteriophage T7 and were used for enzymatic synthesis of modified and isotopically labelled RNA.

Full paper in Portable Document Format: 503a151.pdf

Chemical Papers 50 (3) 151–155 (1996)