ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Extracellular Phosphogalactomannan of Candida mucifera CCY 29-170-1 Strain

A. Kardošová, J. Daniš, and J. Alföldi

Institute of Chemistry, Slovak Academy of Sciences, SK-842 38 Bratislava

Abstract: An extracellular polysaccharide has been recovered from the culture filtrate of the yeast Candida mucifera CCY 29-170-1 strain. Gel filtration of the crude polysaccharide yielded the representative galactomannan composed of (x/%) D-mannose (1.00), D-galactose (0.14), and covalently bound phosphate (7.6 mole %), On the basis of the results of methylation analysis acetolysis. and ¹³C, ¹H, and ³¹P spectral measurements it was concluded that the phosphogalactomannan had an α-(1-->6)-D-mannopyranosyl backbone partially substituted on O-2 with side chains of one, two, or three D-mannosyl units connected by α-(1-->2) linkages. A small portion of the (1-->3) linkages arised from the phosphorylated part of the polymer. D-Galactose was located exclusively at the nonreducing end of side chains. The phosphate was proved to be esterified to primary hydroxyl of side-chain D-mannosyl residues.

Full paper in Portable Document Format: 483a189.pdf

Chemical Papers 48 (3) 189–194 (1994)