ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Influence of pore structure of silica packing on hplc column characteristics

I. Novák, B. Buszewski, J. Garaj, and D. Berek

Polymer Institute, Centre for Chemical Research, Slovak Academy of Sciences, CS-84236 Bratislava

Abstract: The influence of the inner structure of bare silica gel sorbents — espe­cially of their pore volume — on the characteristics of the resulting HPLC columns was studied. The sorbents of various pore characteristics were tested and packed into the columns at optimized column packing con­ditions. The results show that an increase in the sorbent pore volume leads to a slight increase of the column permeability and to a rather pronounced rise of the sample capacity expressed in terms of volume and concentration of a sample. Highly efficient and fairly stable columns can be obtained with strictly spherical silica gels even if their specific pore volume reaches the value of 2 cm³ g^-1.

Full paper in Portable Document Format: 441a31.pdf

Chemical Papers 44 (1) 31–43 (1990)