ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Enhancementof the Thermostability of Candida cylindracea Lipase by Medium Engineering

L. Gubicza, K. Székely, O. Ulbert, and K. Bélafi-Bakó

Research Institute of Chemical and Process Engineering, University of Kaposvár, 8200 Veszprém, Hungary

E-mail: gubicza@mukki.richem.hu

Received: 19 May 2000

Abstract: The possibility to increase the thermostability of the enzyme Candida cylindracea lipase was studied using the medium engineering method. The purpose was to shift the property of the enzyme obtained from mesophilic organism towards thermophilic character and hence widen its application possibilities. Applying the heptane solvent as reaction medium, the relation between the water content of the reaction mixture and the enzyme activity was investigated in the temperature range of 30ºC and 50ºC. It was found that decreasing the water content of the reaction mixture, the activity of the enzyme was reduced, while its thermostability increased. The slower deactivation of the enzyme was explained by the reduced amount of water present, necessary for the denaturation reactions.

Full paper in Portable Document Format: 546aa351.pdf

Chemical Papers 54 (6a) 351–354 (2000)