ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Assessment of role of rosmarinic acid in preventing oxidative process of low density lipoproteins

Andreia Tache, Gabriel-Lucian Radu, and Simona-Carmen Litescu

National Institute of Research and Development for Biological Science, 296 Splaiul Independentei, 060031 Bucharest, Romania

E-mail: gl_radu@chim.upb.ro

Abstract: This study aimed to assess the antioxidant compound effects on oxidisable substrates, using an effective bio-mimetic system based on human low density lipoproteins (LDL). Thermally generated radicals induce LDL oxidative changes to be identified and quantified. The bio-mimetic system thus developed responded linearly to radicals’ concentration over a range of 10⁻⁶-10⁻⁵ mol L⁻¹. Cu²⁺ accentuates lipoperoxidation but, when rosmarinic acid was present, Cu²⁺ produced an unexpected effect, i.e. increased antioxidant efficiency against lipoperoxidation. Rosmarinic acid inhibits production of lipoperoxides by up to 30 % in the absence of Cu²⁺ and up to 70 % in its presence when the rosmarinic acid-to-Cu molar ratio is 1: 1.

Keywords: lipoproteins – peroxyl radicals – hydroxyl radicals – copper ion – antioxidant

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-012-0233-4

Chemical Papers 66 (12) 1166–1170 (2012)