ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Isothiocyanates. XXXVII. New method of determination of natural and synthetic isothiocyanates and glucosinolates

Ľ. Drobnica and V. Knoppová

Department of Microbiology and Biochemistry, Slovak Technical University, 880 37 Bratislava

Abstract: A spectrometric method was developed for detg. isothiocyanates (ITC). It was based on the reaction of ITC with thioglycolic acid by which they are quant. converted into N-substituted thiocarbamoylmercaptoacetates or 3-substituted rhodanines having characteristic uv absorption max. Glucosinolates in plant tissues may thus be detd. after their extn. and enzymic hydrolysis by detg. the liberated ITC. A microsynthesis, paper and thin-layer chromatog. sepn. methods,detection methods, and some phys.-chem. properties of 3-substituted rhodanines are also described.

Full paper in Portable Document Format: 276a799.pdf

Chemical Papers 27 (6) 799–807 (1973)