ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Amino acid composition of dried cultivated lucerne

M. Bačová and J. Zelinka

Slovak Academy of Sciences, Boleraz

Abstract: The amino acid compn. of dried lucerne, cultivated in Bajec, and hydrolyzed in various ways, was detd. by ion-exchange chromatography. After extn. with Et₂O and drying at 105° to const. wt., hydrolysis was accomplished with 6N HCl on a water bath under reflux for 40 hrs. or in partially evacuated ampules at 110° for 20 and 24 hrs. The results were higher using hydrolysis in ampules. For the hydrolysis in ampules, 6N HCl + 6N HCO₂H (1:1), and an 80fold excess of HI were also used. Higher values for cystine and methionine were obtained by oxidn. with peroxyformic acid before hydrolysis; and, in the case of tyrosine, after hydrolysis with HI. The amino acid compn. varied with different cuttings.

Full paper in Portable Document Format: 208a605.pdf (in Slovak)

Chemical Papers 20 (8) 605–610 (1966)