ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Characterization of recombinant antibodies for detection of TNT and its derivatives

Mladen Simonoviíc, Svetlana Milosević-Zlatanović, Nenad Milosavić, Miroslav M. Vrvić, and Branislav Simonović

Institute of General and Physical Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia

E-mail: mladensimonovic@gmail.com

Received: 21 July 2008  Revised: 10 December 2008  Accepted: 16 December 2008

Abstract: Diverse recombinant immunoreagents specific for TNT-derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP-protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT-analog TNP-Tris was 250 fmol or 87 pg mL⁻¹ (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.

Keywords: TNT - assay - antibody - TNP-Tris - recombinant - detection

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-009-0043-5

Chemical Papers 63 (4) 391–398 (2009)