ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

pH-Chromatography of antibiotics. I. Biosynthesis of penicillin

V. Betina

Slovak Technical University, Bratislava

Abstract: By the method of pH-chromatography (CA 53, 9351e) some biosynthetic penicillins were studied. In addn. to pure prepns., the filtrate of the fermentation substrate contg. benzylpenicillin was tested. It was detd. that the side radicals of biosynthetic penicillins had little effect on the character of Rf curves. The relation of Rf values on sepn. coeffs. of benzylpenicillin between H₂O and CHCl₃ at the corresponding pH values was detd. The data of pH chromatograms indicate that there is a possibility of isolation and concn. of antibiotics by applying extn. and reextn.

Full paper in Portable Document Format: 159a661.pdf (in Slovak)

Chemical Papers 15 (9) 661–667 (1961)