ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Extracellular polysaccharides produced by acapsular mutant of Cryptococcus laurentii

J. Dudíková and N. Kolarova

Institute of Chemistry, Slovak Academy of Sciences, SK-845 38 Bratislava, Slovakia

E-mail: chemkola@savba.sk

Received: 29 November 2005  Revised: 24 January 2006  Accepted: 25 January 2006

Abstract: Heterogeneous extracellular polysaccharide galactoxylomannan has been isolated from the culture medium of the acapsulated mutant Cryptococcus laurentii 17-3-6 as the main extracellular polysaccharide (90%). Size-exclusion chromatography of precipitated polysaccharides afforded two molecular fractions with molecular mass 75 kDa and 20 kDa. Mannose, galactose, glucose, and xylose ratios were 1.00: 1.16: 0.04: 0.88 and 1.00: 0.25: 0.07: 0.24, respectively.

Keywords: polysaccharide - galactoxylomannan - isolation - characterization - yeast

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-006-0013-0

Chemical Papers 60 (1) 69–70 (2006)