ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine

Stanislava Rybáková, Michal Rájecký, Jana Urbanová, Kristýna Pěnčíková, Eva Táborská, Raimundo Gargallo, and Petr Táborský

Department of Chemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137, Brno, Czech Republic

E-mail: taborak@email.cz

Abstract: Benzo[c]phenanthridine alkaloid sanguilutine was extracted and purified from the dried roots of Sanguinaria canadensis. The interaction of the positively charged iminium form of alkaloid with double-stranded DNA oligonucleotides was studied using luminescence spectroscopy. The results showed that the interaction with various double-stranded oligonucleotides was not specific to A-T or G-C base pairs; also, no preference was found for either homogeneous or heterogeneous base composition of strands. The association constants were calculated to be in the range of (1.31–14.36) × 10⁵ M⁻¹. The luminescence intensity response at 610 nm to low concentrations of double-stranded DNA was found to be linear and can potentially be used for the fluorometric quantification of DNA. The limit of detection was estimated to be 120 ng mL⁻¹ of DNA (calculated by 3σ method).

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-013-0340-x

Chemical Papers 67 (5) 568–572 (2013)