ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Development of an effective extraction process for coenzyme Q₁₀ from Artemia

Thitima Rujiralai, Nirundorn Raekasin, Wilairat Cheewasedtham, and Chonlatee Cheewasedtham

Department of Chemistry and Centre of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

E-mail: thitima.r@psu.ac.th

Abstract: A method for extracting coenzyme Q₁₀ (CoQ₁₀) from Artemia was developed. 1 g of fresh Artemia was incubated with 75 % acetic acid at (30 ± 2)°C for 24 h, followed by three consecutive extractions with a mixture of 5 mL of hexane and 5 mL of ethanol, then analysis by a validated high-performance liquid chromatography with a diode-array detector. The calibration curve for CoQ₁₀ was linear in a range of 1–50 μg mL⁻¹. The limits of detection and quantification were 0.3 μg mL⁻¹ and 1.1 μg mL⁻¹, respectively. Mean recoveries were 94–100 % with a high precision of below 10 %. The method developed was found to be simple, efficient and the time required for releasing CoQ₁₀ from Artemia was short. The method provides not only low energy consumption but is also practical for industrial applications.

Keywords: coenzyme Q10 – Artemia – extraction – high-performance liquid chromatography

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-014-0558-2

Chemical Papers 68 (8) 1041–1048 (2014)