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Determination of catechin and epicatechin in the peel of apple varieties resistant and non-resistant to apple scab

Marek Gołębiowski, Edmund Maliński, Małgorzata Szankin, Mariusz Marszeniuk, Monika Paszkiewicz, and et al.

Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland

 

E-mail: goleb@chem.univ.gda.pl

Abstract: Catechin and epicatechin were analysed in the peel of six apple cultivars (three resistant and three non-resistant to apple scab). Two methods of analytical sample preparation following extraction were tested: solid phase extraction and column separation with Sephadex LH-20 coupled to a differential refractometric detector. Prior to GC and GC-MS analyses, these compounds were silylized. This permitted co-injection with standards and the comparison of retention values and mass spectra with those recorded for standards. The content of catechin and epicatechin in apple peel is discussed in relation to the resistance of apple trees to scab.

Keywords: catechins - apple scab - refractometric detector - GC - GC-MS

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-010-0067-x

 

Chemical Papers 64 (6) 729–733 (2010)

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