ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Electrochemical enzymatic biosensors based on metal micro-/nanoparticles-modified electrodes: a review

Nina D. Dimcheva and Elena G. Horozova

Department of Physical Chemistry, Plovdiv University, 24, Tsar Assen Street, BG-4000 Plovdiv, Bulgaria

E-mail: nina.dimcheva@uni-plovdiv.net

Abstract: The functions of metal structures of micro- or nano-dimensions in the sensing mechanisms of amperometric enzyme-based biosensors are considered in the light of the principles of detection of the latter. The applications of metal mono- or bimetallic nanoparticles-modified materials as catalytic electrodes in the fabrication of first-generation and the role which metal nanoparticles play in promoting or enhancing the electron transfer rates in third-generation electrochemical biosensors are reviewed. Some examples of gold NPs functionalised with enzymes via gold-thiol chemistry as a strategy for enzyme immobilisation and spatial orientation when developing amperometric biosensors are also discussed.

Keywords: nanoparticles – biosensors – electrocatalysis – direct electron transfer (DET)

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2015-0011

Chemical Papers 69 (1) 17–26 (2015)