ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode

Cristina Gutierrez-Sanchez, Sergey Shleev, Antonio L. De Lacey, and Marcos Pita

Instituto de Catalisis y Petroleoquimica, CSIC, C/Marie Curie 2, L10, 28049 Madrid, Spain

E-mail: marcospita@icp.csic.es

Abstract: The response of low-density graphite electrodes hosting Trametes hirsuta laccase in a direct electron transfer regime is presented for real-time analysis of O₂ concentrations. The use of contrasting immobilisation methods developed for biocathodes affords good reproducibility and reliability of the amperometric biosensor, which shows a limit of detection below 1 μM and a sensitivity slightly higher than 60 nA cm⁻² M⁻¹.

Keywords: oxygen biosensor – direct electron transfer – laccase – bioelectrocatalysis

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-014-0595-x

Chemical Papers 69 (1) 237–240 (2015)