ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Physicochemical aspects of Trichosporon cutaneum CCY 30-5-10 adhesion and biofilm formation potential on cellophane

Jitka Dostálková, Gita Procházková, Vladimír Jirků, Lucie Křiklavová, Tomáš Lederer, Miloslav Suchánek, and Tomáš Brányik

Institute of Chemical Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic

E-mail: tomas.branyik@vscht.cz

Abstract: This paper focuses on the adhesion and biofilm formation potential of cellulolytic yeast Trichosporon cutaneum CCY 30-5-10 on solid cellophane from a novel perspective. First, physicochemical characterisation of the cells and carrier (cellophane) was performed to evaluate the effect of different culture media (complex vs mineral) on yeast cell adhesion. (Un)favourable adhesion conditions were predicted using the thermodynamic approach. Next, the ability of the cells to colonise the carrier under the above conditions was quantified and the biofilm structure was characterised using image analysis. The approaches described were found suitable to predict and experimentally verify favourable (cell-solid) adhesion, i.e. the hydrophobic and low electron-donor nature of cellophane together with hydrophobic cells (obtained when cultivated in a complex culture medium) were found to have a major impact in defining successful yeast adhesion with subsequent biofilm formation.

Keywords: cellophane – Trichosporon cutaneum – biofilm formation – adhesion – physicochemical surface characterisation – thermodynamic model

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2015-0046

Chemical Papers 69 (3) 425–432 (2015)