ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Spectroscopy studies of sandwich-type complex of silver(I) co-ordinated to nuclear fast red and adenine and its analytical applications

Yi Cui, Yi-Jun Gong, Li Su, Cheng-Ke Wu, Sheng-Bin Pang, and Su-Ling Feng

Collaborative Innovation Centre of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, 453007 Xinxiang, China

E-mail: slfeng@htu.cn

Abstract: A novel fluorometric method for the determination of adenine was proposed, based on the fluorescence quenching of a nuclear fast red-silver complex as a small molecule optical probe in the presence of adenine. The characteristic spectra of absorption, resonance Rayleigh scattering (RRS) and the fluorescence of the system were analysed. Adenine, Ag⁺ and nuclear fast red formed a sandwichtype complex. The composition of the compound and the mechanisms of fluorescence quenching were discussed. The optimal conditions for the reaction and the effects of co-existing substances were also investigated. The quenched fluorescence intensity was proportional to the concentration of adenine in the range of 0.03-0.90 μg mL^-1, with the limit of detection of 0.023 μg mL^-1. The method was applied satisfactorily to the determination of adenine in synthetic samples, herring sperm DNA, human sera and vitamin B4 tablets with recoveries from 95.3 % to 105 %.

Keywords: sandwich-type complex – adenine – nuclear fast red – Ag+ – fluorescence quenching

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2015-0056

Chemical Papers 69 (4) 510–517 (2015)