ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Characterization and optimization of mesoporous magnetic nanoparticles for immobilization and enhanced performance of porcine pancreatic lipase

Yong-Bo Shao, Tao Jing, Jing-Zhi Tian, Yong-Jie Zheng, and Ming-Hui Shang

College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China

E-mail: jtkr@163.com

Abstract: In this paper, Fe₃O₄ nanoparticles coated with mesoporous silica were prepared successfully, noted as Fe₃O₄ at the mobile composition of matter No. 41 (MCM-41). Also, Fe₃O₄ at MCM-41 was grafted by both 3-aminopropyltriethoxysilane (APTS) and 3-chloropropyltriethoxysilane (CPS), noted as Fe₃O₄ at MCM-41/APTS and Fe₃O₄ at MCM-41/CPS. The compounds were characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometry, thermogravimetry and N₂ adsorption/desorption. Then, the enzyme, porcine pancreas lipase (PPL), was immobilized onto these modified nanoparticles by covalent attachment, physical adsorption and cross-linking, noted as Fe₃O₄ at MCM-41/CPS-PPL, Fe₃O₄ at MCM-41-PPL and Fe₃O₄ at MCM-41/APTS-PPL, respectively. The results showed that Fe₃O₄ at MCM-41/CPS was the best nanomaterial for PPL immobilization, exhibiting enhanced immobilization efficiency (maximum 96 %), maximum relative activity (up to 96 %), high stability and reusability (83 % 56 days and 86.7 % ten cycles). Additionally, it offered some other advantages, such as easy recycling and reuse, complying with the trend of green chemistry. Therefore, Fe₃O₄ at MCM-41/CPS in combination with a relevant method can be proposed for commercial applications.

Keywords: Fe3O4 nanoparticles – porcine pancreas lipase – covalent attachment – physical adsorption – cross-linking

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2015-0142

Chemical Papers 69 (10) 1298–1311 (2015)