ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Repetitive inductions of bioluminescence of Pseudomonas putida TVA8 immobilised by adsorption on optical fibre

Jakub Zajíc, Milan Bittner, Tomáš Brányik, Andrey Solovyev, Stanislav Šabata, Gabriela Kuncová, and Marie Pospíšilová

Faculty of Biomedical Engineering, Czech Technical University in Prague, 27201 Kladno 2, Czech Republic

E-mail: kuncova@icpf.cas.cz

Abstract: Physico–chemical models of the interactions of cells with solid surfaces, which use contact angles and zeta potentials, indicated more facile adsorption of cells of Pseudomonas putida TVA8 on the quartz surface after its treatment with 3-aminopropyltriethoxysilane (APTES). A whole-cell optical fibre sensor of toluene was prepared by the adsorption of P. putida TVA8, bacteria producing light in contact with toluene on the wider end of APTES-treated quartz tapered optical fibre. The results of the measurements of luminescence from both sides of the layer of adsorbed cells were compared. Over the 135 days trial, the fibre biosensor was repetitively induced with toluene solution (26.5 mg L⁻¹) 68 times. The intensities of bioluminescence gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of bioluminescence induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene.

Keywords: whole-cell biosensor – bioluminescent bioreporter – Pseudomonas putida TVA8 – optical fibre sensor – adsorbed microorganisms – water contamination – BTEX

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2016-0031

Chemical Papers 70 (7) 877–887 (2016)