ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Progress in emerging techniques for characterization of immobilized viable whole-cell biocatalysts

Marek Bučko, Alica Vikartovská, Andrea Schenkmayerová, Ján Tkáč, Jaroslav Filip, Dušan ChorvátJr., Vilém Neděla, Marion B. Ansorge-Schumacher, and Peter Gemeiner

Slovak Academy of Sciences, Bratislava, Slovakia

E-mail: Peter.Gemeiner@savba.sk

Abstract: Proper understanding of viable cell physiology during biocatalytic processes, progress in development of biocompatible immobilization techniques, as well as introduction of new non-invasive characterization techniques are the main prerequisites for the successful development of novel whole-cell biocatalysts. Selection of the optimal immobilized viable cell biocatalyst is possible when standardised characterization techniques are employed. The development of these techniques requires advanced analytics and a multidisciplinary effort. This review provides a summary on progress in development of characterization methods and emerging tools for determination of morphology, physiology, and biocatalytic efficiency of immobilized viable whole cells.

Keywords: Bioelectrocatalysis; Imaging techniques; Immobilized whole-cell biocatalyst; Multienzyme cascade reactions; Online kinetics

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-017-0243-3

Chemical Papers 71 (11) 2309–2324 (2017)