ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

In vitro effect of ethyl acetate, butanol and water extracts of Juniperus excelsa Bieb. on angiotensin-converting enzyme purified from human plasma

Zehra Basi, Nalan Turkoglu, Vedat Turkoglu, and Fatih Karahan

Van Yüzüncü Yıl University, Van, Turkey

E-mail: zehrabasi@yyu.edu.tr

Abstract: Angiotensin-converting enzyme (ACE) was purified from human plasma by affinity chromatography. Effect of ethyl acetate, butanol and water extracts of Juniperus excelsa Bieb. (J. excelsa) fruits on purified ACE activity was investigated. ACE was purified 3659-fold with a specific activity of 1350 EU/mg protein from human plasma. The purity and molecular weight of ACE were determined by SDS-PAGE, and two bands 60 kDa and 70 kDa are seen on the gel. Ethyl acetate extract of J. excelsa fruits showed an activation effect on human plasma ACE activity. Butanol and water extracts of J. excelsa fruits showed an inhibition effect on ACE activity. IC₅₀ values for butanol and water extracts of J. excelsa fruits were calculated to be 2.858 mg/mL and 5.790 mg/mL, respectively. Lisinopril was used to be reference inhibitor. Type of inhibition for all inhibitors was designated as non-competitive. IC₅₀ value and K_i constant for lisinopril were determined to be 0.781 nM and 0.662 nM, respectively. These results show that butanol and water extracts of J. excelsa plant may have an ACE inhibitor potency.

Keywords: Angiotensin-converting enzyme ; Purification ; Juniperus excelsa ; Inhibition ; Extract 

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-019-00806-w

Chemical Papers 73 (10) 2525–2533 (2019)