ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Simultaneous analysis of three catecholamines by a kinetic procedure: comparison of prediction performance of several different multivariate calibrations

Yongnian Ni, Yao Gu, and Serge Kokot

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047 China

E-mail: ynni@ncu.edu.cn

Abstract: A rapid kinetic method for the simultaneous determination of levodopa, dopamine, and dobutamine was examined and developed. It was based on a consecutive reaction of a reduction of Cu(II) to Cu(I) by catecholamines, followed by the complexation of Cu(I) with neocuproine to form a yellow product in an acetic acid-acetate buffer. Spectrophotometric data were recorded at 453 nm (wavelength at the yellow complex absorption maximum) for 300 s. Linear calibrations were obtained in the concentration ranges of (0.08–1.44) × 10⁻⁵ mol L⁻¹, (0.08–1.44) × 10⁻⁵ mol L⁻¹, and (0.16–1.44) × 10⁻⁵ mol L⁻¹ for levodopa, dopamine, and dobutamine, respectively. A variety of multivariate calibration models was developed for simultaneous analysis of the three analytes; while most models produced satisfactory prediction results for synthetic samples, the hybrid linear analysis method was arguably the best-performing (relative prediction error, RPET = 6.6 %). The proposed method was applied to an analysis of spiked rabbit serum samples and the results showed good agreement with the high performance liquid chromatography measurements.

Keywords: catecholamines – multivariate calibrations – hybrid linear analysis – kinetics

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-011-0090-6

Chemical Papers 65 (6) 782–791 (2011)