ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Simultaneous trace monitoring of prokinetic drugs in human plasma using magnetic dispersive micro-solid phase extraction based on a new graphene oxide/metal–organic framework-74/Fe₃O₄/polytyramine nanoporous composite in combination with HPLC

Fatemeh Pourbahman, Mohsen Zeeb, Amirhossein Monzavi, and Seyed Saied Homami

South Tehran Branch, Islamic Azad University, Tehran, Iran

E-mail: zeeb.mohsen@gmail.com

Abstract: In this work, graphene oxide/metal–organic framework-74/Fe₃O₄/polytyramine (GO/MOF-74/Fe₃O₄/PTy) nanoporous composite was synthesized as a sustainable sorbent for magnetic dispersive micro-solid phase extraction (MD-µ-SPE) of prokinetic drugs. The new nanosacle sorbent in combination with high performance liquid chromatography–ultraviolent detection (HPLC–UV) was applied for simultaneous trace quantification of target drugs in human plasma. MOF-74 and PTy were used as surface modifiers to improve the properties of GO nanosheets, such as surface area-to-volume ratio, adsorption capacity, hydrophobic interactions and selectivity. A composite of GO/MOF-74/Fe₃O₄ was fabricated and then an oxidative polymerization of tyramine was performed on the surface of sorbent using horseradish peroxidaze (HRP) enzyme as a catalyst. The characterization of the hybrid sorbent was evaluated using scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and Fourier transform-infrared (FT-IR) spectroscopy. The limit of detections (LODs, S/N = 3) for domperidone (DOM) and itopride (ITP) were 0.4 and 1.1 ng mL⁻¹, respectively. Notable linearity (0.995 ≥ r² ≥ 0.991) and practical dynamic concentration ranges of 1.5–1100.0 ng mL⁻¹ and 4.0–1750.0 ng mL⁻¹ were obtained for DOM and ITP, respectively. Intra-assay (≤ 8.6%, n = 12) and inter-assay (≤ 9.0%, n = 12) precisions along with appreciable accuracies (≤ 9.3%) demonstrated satisfactory performance of the current method. Ultimately, this approach was utilized for trace monitoring of DOM and ITP in human plasma after low dose administration and some pharmacokinetic features were investigated in detail.

Keywords: Magnetic dispersive micro-solid phase extraction ; Metal–organic framework ; Graphene oxide ; Polytyramine ; Prokinetic drugs ; High-performance liquid chromatography 

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-019-00855-1

Chemical Papers 73 (12) 3135–3150 (2019)