ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Electrochemical quantification of pyridoxine (VB₆) in human blood from other water-soluble vitamins

R. Rejithamol and S. Beena

Department of Chemistry, Amrita School of Arts & Sciences, Amrita Vishwa Vidyapeetham, Kollam, India

E-mail: beenas@am.amrita.edu

Received: 5 September 2019  Accepted: 31 December 2019

Abstract:

For the first time reporting a pencil graphite electrode modified with gold nanoparticles and non-conducting polymeric film of o-aminophenol for the selective determination of pyridoxine (VB₆) from other water-soluble vitamins in human blood. The fabricated electrode was characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, and attenuated total reflection infrared spectroscopy. Voltammetric response of VB₆ on the fabricated electrode was investigated out using cyclic and differential pulse voltammetric techniques. Under optimized conditions, an irreversible oxidation peak at + 0.632 V was observed which corresponds to the oxidation of VB₆. The oxidation peak current and the pyridoxine concentration were found to be linear in the range from 5 to 200 μM with a detection limit of 0.30 μM. The sensitivity of the proposed electrode was calculated to be 3.373 μA/μM/cm² with excelled reproducibility and stability. The electrode is suitable for the routine analysis of VB₆ in human blood samples as it highly selective from other water-soluble vitamins.

Graphic Abstract:

Keywords: Pyridoxine; o-Aminophenol; Gold nanoparticles; Pencil graphite electrode

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-019-01049-5

Chemical Papers 74 (6) 2011–2020 (2020)