ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
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Influence of selected biowaste materials pre-treatment on their anaerobic digestion

Alžbeta Takáčová, Tomáš Mackluľak, Miroslava Smolinská, Miroslav Hutňan, and Petra Olejníková

Slovnaft VÚRUP, a.s., Vlčie Hrdlo, 820 01 Bratislava, Slovakia



Abstract: The topic of this study is the pre-treatment of substrates for anaerobic digestion. Two different substrates of algae Scenedesmus subspicatus (SAG 86.81), Chlorella kessleri (LARG/1) and foliage of Prunus serrulata were subjected to anaerobic digestion. A mixture of commercially available cellulolytic enzymes (Analytical science s.r.o., Modra, Slovakia) was used for anaerobic treatment of algae while the foliage of Prunus serrulata was pre-treated by lignolytic fungi. The highest production of methane per mass of volatile solids was reached with untreated Chlorella kessleri at (0.59 ± 0.04) L g−1. The addition of cellulolytic enzymes did not increase the production of methane from the algal substrate; however, a faster substrate degradation and thus also higher speed of methane production at the beginning of cultivation was achieved. After foliage pre-treatment by fungal isolate Pleurotus pulmonarius, isolated from natural habitats, the methane production increased five times. In this way we were able to speed up the processes of biological degradation of ligno-cellulose materials and thereby to increase the production of methane. Our results show the possibility of using algae as a suitable substrate for biogas production. On the other hand, also aerobic pre-treatment of foliage (Pleurotus pulmonarius) presents a successful way for speeding up the degradation of ligno-cellulose waste leading to increased methane yields.

Keywords: methane – algae – leaves – elementary analysis – Micro-Oxymax

Full paper is available at

DOI: 10.2478/s11696-011-0107-1


Chemical Papers 66 (2) 129–137 (2012)

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