ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Gelatin–rosin gum complex nanoparticles: preparation, characterization and colon targeted delivery of 5-fluorouracil

Sneha Joshi and Vandana Singh

Department of Chemistry, University of Allahabad, Allahabad, India

E-mail: singhvandanasingh@rediffmail.com

Received: 9 April 2020  Accepted: 26 May 2020

Abstract:

Abstract

Gelatin-rosin gum complex nanoparticles (GGR_NPs) have been synthesized by complex coacervation method. The particles were extensively characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and particle size analysis based on dynamic light scattering (DLS). The GGR_NP3 were stable and their average particle size was  ~ 153 nm. GGR_NPs were evaluated as the carrier matrix for 5-fluoro uracil (5-FU), and the release behavior of 5-FU was examined by swelling and in vitro dissolution tests in simulated gastrointestinal fluid (SGF) for first 2 h followed by 14 h in simulated intestinal fluid (SIF). About 71% drug release was witnessed (SGF = 21%; SIF = 50%) over a time span of 16 h, in contrast to native gelatin or rosin gum where under identical conditions the release exhausted within 7 h and 10 h, respectively. The release profile followed the first-order kinetics and the diffusion exponent (n) values obtained from the Korsemeyer−Peppas model ranged between 0.5 < n < 0.8 (both in SGF and SIF), which indicated non-Fickian diffusion mechanism. A549 cell line was used to carry out MTT assay test for investigating the cell toxicity of the drug-loaded GGR_NP3 (D GGR_NP3) where DGGR_NP3 exhibited greater toxicity as compared to GGR_NP3.

Graphic abstract

Keywords: Gelatin; Rosin gum; Glutaraldehyde; 5-Fluoro uracil; Drug delivery

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-020-01231-0

Chemical Papers 74 (12) 4241–4252 (2020)