ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Synthesis, characterization and DNA binding properties of 3,6-bis[(alkylamino)ureido]acridines

Danica Sabolová, Simona Sovová, Ladislav Janovec, Eva Timko, David Jager, and Jana Tóthová

Department of Biochemistry, Institute of Chemistry, Faculty of Science, P. J. Šafárik University, Košice, Slovak Republic

E-mail: danica.sabolova@upjs.sk

Received: 25 August 2022  Accepted: 19 January 2023

Abstract:

New four 3,6-bis[(alkylamino)ureido]acridines were synthesized and thoroughly characterized using NMR spectra. In vitro DNA binding studies of acridines carried out employing absorption, fluorescence and circular dichroism measurements revealed their binding into DNA via intercalation. The UV–Vis absorbance data were analysed to obtain the binding constant (K_b). K_b values were found in the range of 4.46 − 6.73 × 10⁴ M⁻¹. The DNA viscosity measurements were also performed to confirm the binding mode of the investigated acridines.

Graphical abstract

Keywords: Acridine; Proflavine; Intercalation; Viscosimetry

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-02694-7

Chemical Papers 77 (6) 3155–3162 (2023)