ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Optimization of Cultivation Conditions for Production of Fructosyltransferase by Aureobasidium pullulans

M. Vandáková, Z. Platková, M. Antošová, V. Báleš, and M. Polakovič

Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-812 37 Bratislava

E-mail: marcela.vandakova@stuba.sk

Received: 1 April 2003

Abstract: The investigation of optimal conditions for the fructosyltransferase (FTase) production by Aureobasidium pullulans CCY 27-1-94 was carried out in shaken flasks. The effect of four medium components was investigated by varying their content compared to the reference medium composition. It was found that none of the investigated components had a very strong effect either on the cell growth or the FTase production. It was, however, disadvantageous to decrease the content of the components below the values in the reference medium. The influence of initial sucrose concentration and cultivation time was investigated in the second stage using a rotatable central composite design where the sucrose concentration was 208-491 g dm⁻³ and the cultivation time was from 26 to 94 h. The sucrose content did not influence the total production of FTase but high sucrose concentrations resulted in high specific activities of the cells. The cultivation time had a positive effect on the fructosyltransferase production up to 40|60 h but it decreased the specific enzyme activity. The effect of the composition of the cultivation medium on the total costs of fructooligosaccharide production was also evaluated.

Full paper in Portable Document Format: 581a15.pdf

Chemical Papers 58 (1) 15–22 (2004)