ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Effect of Sucrose Concentration and Cultivation Time on Batch Production of Fructosyltransferase by Aureobasidium pullulans CCY 27-1-1194

M. Antošová, M. Polakovič, M. Slovinská, A. Madlová, V. Illeová, and V. Báleš

Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-812 37 Bratislava

E-mail: antosova@chtf.stuba.sk

Received: 28 March 2002

Abstract: The e ect of the initial sucrose concentration (50 g dm⁻³, 200 g dm⁻³, and 350 g dm⁻³) and time of cultivation (1 d, 2 d, and 4 d) on the fructosyltransferase production by Aureobasidium pullulans CCY 27-1-1194 in shaken flask cultivations was investigated. It was found that the overall fructosyltransferase production depended strongly on the initial sucrose concentration when the highest value of enzyme activity (130 000 U dm⁻³) was achieved at 350 g dm⁻³ in the fourth cultivation day. The maximum of speci c activity per dry cell mass was achieved at the same sucrose concentration, but already in the second day. Since the second cultivation day, the ratio of the enzyme activity contained in cells and released into medium was 1.3—1.6, independently of sucrose concentration. In order to discuss the fructosyltransferase production in relation to cell growth and state of the culture, the sucrose conversion, produced amount of fructooligosaccharides and organic acids, and the yield factor of biomass on substrate, were determined as well.

Full paper in Portable Document Format: 566a394.pdf

Chemical Papers 56 (6) 394–399 (2002)