ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Use of green fluorescent proteins for in vitro biosensing

Zbynek Heger, Ondrej Zitka, Zdenka Fohlerova, Miguel Angel Merlos Rodrigo, Jaromir Hubalek, Rene Kizek, and Vojtech Adam

Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic

E-mail: vojtech.adam@mendelu.cz

Abstract: Due to the considerable stability of green fluorescent proteins and their capacity to be readily permutated or mutated, they may be exploited in multiple ways to enhance the functionality of in vitro biosensors. Many possibilities, such as the formation of chimeras with other proteins or antibodies, as well as Förster resonance emission transfer performance, may be used for the highly sensitive and specific detection of the target molecules. This review considers the great potential of green fluorescent proteins as the fluorescent probing or recognition biomolecule in various in vitro biosensors applications, as well as obstacles associated with their use.

Keywords: Aequorea victoria – biosensor – chimera – emission – Förster resonance emission transfer

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-014-0588-9

Chemical Papers 69 (1) 54–61 (2015)