ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

The isolation of tyrosine sad leucine from corn gluten

D. Ivančenko, P. Zajac, and A. Oravcová

Slovak Technical University, Bratislava

Abstract: A refined corn gluten contg. 94.5% dry matter and 70.5% protein in the dry matter was hydrolyzed for 7 hrs. at the boiling point with 10-fold excess 8N H₂SO₄, and the ppt. sepd. by filtration. The pH of the filtrate was adjusted to 5.7 (isoelectric point of tyrosine) with satd. Ba(OH)₂ and cryst. tyrosine isolated. The pH of the filtrate was adjusted to 5.9 (isoelectric point of leucine) and concd. at 65-75° to 1/5 of the original vol. The isolated crystals contained the leucine as well as glutamic and aspartic acids and alanine.

Full paper in Portable Document Format: 182a148.pdf (in Slovak)

Chemical Papers 18 (2) 148–151 (1964)